foxm1 molecular weight

[Lot No. The SUMO protein has a molecular weight of about 11 kDa and is an inactive precursor molecule at the beginning. ... FOXM1 has been demonstrated … Journal. Plantazolicin (PZN) was predicted by bioinformatics to be an excreted metabolite from the soil-dwelling microbe Bacillus amyloliquefaciens FZB42. FOXM1 is a molecular determinant of the mitogenic and invasive phenotype of anaplastic thyroid carcinoma. Biochim Biophys Acta (BBA) – Gene Reg Mech, 2014, 1839 (11): 1316-22. Tissue Specificity: Q08050 FOXM1_HUMAN: Expressed in thymus, testis, small intestine, colon followed by ovary. OBJECTIVE—This study was designed to determine whether the transcription factor FoxM1 was required for regeneration of β-cell mass via proliferation and/or neogenesis in the adult after 60% partial pancreatectomy (PPx). Over-FoxM1 expression upregulates the ability of JAR cells to migrate and invade and vice versa. Molecular Weight: 437.43. The forkhead box M1 (FOXM1) transcription factor plays crucial roles in regulating the proliferation, differentiation, and transformation of cells. Price: 10 µg/$461; 25 µg/$611. Blocks bacterial protein synthesis by inhibition of mRNA-tRNA translocation by GTPase elongation factor G (EF-G), EF-TU(GTP)-catalyzed aa-tRNA delivery as well as the activity of initiation factor-1 (IF-2). ALIX is an exosomal specific membrane protein and a component of ESCRTiii complex and is involved in the biogenesis of exosomes [].It was expressed in exosomes derived from all the eight cell lines at the molecular weight of 102 kDa (Fig. Aurora B is regulated by the mitogen-activated Article CDC2 is an important driver of vascular smooth muscle cell proliferation via FOXM1 and PLK1 in pulmonary arterial hy-pertension. FOXM1 (Homo sapiens) Host rabbit. Upon the addition of 150ng of FOXM1, we observed an increase in the signal intensity between 3 and 4ppm indicating the binding of 339163-65-4: Storage: 3 years-20°C: powder: 2 years-80°C: in solvent Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. FOXM1 was detected by western blot analysis and a higher molecular weight form was observed consistent with SUMOylation of FOXM1. Purity of exosomes were further verified by immunoblotting for exosomal specific and non-specific proteins. Cell and Tissue Research – Springer Journals The MA5-17078 immunogen is purified recombinant fragment of human FOXO1 expressed in E. Coli. Immunogen. Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Stable for one year after … Monoclonal Antibody for studying FOXM1 in the research area. MW, molecular weight. In the current study, we have identified sumoylation as a novel mechanism of FOXM regulation in breast … high molecular weight proteins, such as FOXM1. However, the precise role and molecular mechanism responsible for the aggressive action of FOXM1 in … Preparing Stock Solutions for Thiostrepton. Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). Purification: Purified by Protein A. The fraction numbers, elution of molecular weight markers with arrows, and PKM2 monomer/polymers are indicated. Western analysis of FOXM1 expression indicated the presence of multiple species of this protein, and IP pull-down using a TEAD1 antibody clearly showed preferential binding of TEAD1 to the higher-molecular-weight form. Proteasome activity alters the phosphorylation status of FoxM1. The Role of Epithelial Mesenchymal Transition Markers in Thyroid Carcinoma Progression. This method allows for the investigation of small molecule binding to high molecular weight proteins, such as FOXM1. (B) The lysates were pulled down with GST or GST-TUBE and analyzed with the antibodies against FOXM1 and polyubiquitin K-48-linkage (UbK48). PCRP-FOXM1-1H4 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-FOXM1-1H4) Storage and Handling Recommendations Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. A small-molecule compound, Robert Costa Memorial drug-1 (RCM-1), has been recently identified from high-throughput screen as an inhibitor of FOXM1 in vitro and in mouse model of allergen-mediated … 7 Genetic evaluation of the PZN biosynthetic pathway, molecular weight determination, and confirmation of antibiotic activity were reported in early 2011. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website. I used antibodies in WB in dilution 1:1000. The first column is a protein marker to determine the molecular weight of each protein band. In the pancreas, proglucagon was found to be cleaved to produce two fragments, mature glucagon and a 10,000-MW second protein. FOXM1 is most abundant in thymus, testis, small intestine and colon. The Dynabeads-Ab-Antigen complexes were eluted according to the manufacturer’s instructions, separated on SDS-PAGE, and analyzed by western blotting at the molecular weight of FOXM1 using rabbit polyclonal anti-phospho-tyrosine (#9411, Cell Signaling) and rabbit polyclonal anti-cyclin D1 antibodies. To investigate the functional role of forkhead box M1 (FoxM1) in the mechanism of endothelial repair, we generated endothelial cell– restricted FoxM1-deficient mice (FoxM1 CKO mice). Molecular Weight: 1648.84. For more info on FOXM1, check out the FOXM1 Infographic We have 30,000+ of these available, one for each gene! (F) FOXM1 levels in MDA-MB-468 TNBC cells after 24 h treatment with ICG-001 or DMSO control. The gene encoding human FOXM1 maps to chromosome 12p13. The transcription element that restricts FOXM1 expression to proliferating cells is located 300 bp upstream of the start codon. Expression of Foxm1 is found in a variety of distinct human cancers including hepatocellular carcinomas, intrahepatic cholangiocarcinomas, basal … Overexpression of FOXM1 is associated with a variety of aggressive solid carcinomas, including bladder cancer. In the present study, we found the FOXM1 protein inside the mitochondria. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. FOXM1, also known as FKHL16, MPP2 or Trident, is primarily expressed in proliferating cells. The molecular weight of the PCR products is shown on the right. Molecular Weight: Observed Mol.Wt. Clin Cancer Res 23(5):1323-1333, 2017. e-Pub 2016. FOXM1 is a molecular determinant of the mitogenic and invasive phenotype of anaplastic thyroid carcinoma Roberto Bellelli1,2, Maria Domenica Castellone1,2, Ginesa Garcia-Rostan3, Clara Ugolini4, Carmelo Nucera5, Peter M Sadow6, Tito Claudio Nappi1,2, Paolo Salerno1,2, Maria Carmela Cantisani1,2, Fulvio Basolo4, Tomas Alvarez Gago3, Giuliana Salvatore7 and Massimo … Reacts with: Mouse, Human. [28] Y. Y. Clonality: Polyclonal. high molecular weight pr oteins, such as FOXM1. In this infographic you will see the following information for FOXM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, … Product Number. A cell-permeable thiostrepton family macrocyclic peptide antibiotic that inhibits bacterial protein synthesis by interfering with rRNA binding. The transcription element that restricts FOXM1 expression to proliferating cells is located 300 bp upstream of the start codon. The first column is a protein marker to determine the molecular weight of each protein band. The molecular mechanisms of SioA on enhancing the cytotoxic activity of 5-FU via suppression of both FOXM1 and TS expression and function are demonstrated. The first and last column is a protein marker to determine the molecular weight of each protein band. Initial experiments indicated that at 2mM concentrations, hon-okiol aggregated, generating an STD signal without the addition of the protein (Fig. External resources. The following data is based on the product molecular weight 424.569 Batch specific molecular weights may … Forkhead box M1 (FOXM1), an oncogenic transcription factor associated with aggressiveness and highly expressed in many cancers, is an emerging therapeutic target. Initial. Using site-directed mutagenesis, we generated FOXM1 mutant proteins that localized to distinct cellular compartments, uncoupling the nuclear and mitochondrial functions of FOXM1. Clonality polyclonal. FOXM1. Affinity Purified. FOXM1 Protein LS-G12762 is a Recombinant Rat FOXM1 produced in E. coli aa 209-460 with His-T7 tag(s). Targeting FOXM1 sensitizes breast , gastric, and ovarian cancer cells to chemotherapy, as well as lung cancer and B cell leukemia to tyrosine kinase inhibition. The gene encoding human FOXM1 maps to chromosome 12p13. Product Number Product Description SDS; 42575: Western Blot: FoxM1 Antibody [NBP2-16519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody diluted at 1:500. Characteristics. Rabbit monoclonal [EPR17379] to FOXM1. Synonyms: FOXM1 Inhibitor III, FDI-6 - Calbiochem. The function of FOXM1 independent of nuclear transcription is unknown. In this study, we aim to clarify the molecular basis for FOXM1 … The molecular weight of the PCR products is shown on the right. Molecular Weight (predicted) of FOXM1C isoform: 84 kDa. Genome-wide microRNA analysis identifies miR-188-3p as novel prognostic marker and molecular factor involved in colorectal carcinogenesis. 1/500 … By Mariangela Zane and Marco Bonanno. Plays a role in the control of cell proliferation. We observed a higher molecular weight band in the DMSO-treated conditions, which was absent in the immunoprecipitate of the MG132 treated cells . (E) The PK activity of individual fractions from HeLa cells that were ectopic expression with vector, FOXM1A, or FOXM1D. FOXM1 mediates resistance to genotoxic agents, such as γ-irradiation and epirubicin, through regulating genes (10, 11) involved in DNA damage repair. Inhibits expression of FOXM1 target genes. [27] S. Zona et al. check them out. : 84kDa(Calculated).. Subcellular Location: Nucleus. What is the molecular weight of the protein? Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. For more info on FOXM1, check out the FOXM1 Infographic We have 30,000+ of these available, one for each gene! FOXM1 information: Molecular Weight: 84283 MW; Subcellular Localization: Nucleus; Tissue Specificity: Expressed in thymus, testis, small intestine, colon followed by ovary. 263C2a-1] Predicted molecular weight: 88 kDa FOXM1 Antibody (aa713-763) LS-C288326 Antibody: FOXM1 Rabbit anti-Human Polyclonal (aa713-763) Antibody . 1/500 - 1/2000. MA5-17078 targets FOXO1 in ChIP, IHC, IF, and WB applications and shows reactivity with Human and Mouse samples. In this infographic you will see the following information for FOXM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, … 1e-I). Our investigation of FoxM1 should provide new insights into the molecular mechanisms of EVT invasion. (1 u is equal to 1/12 the mass of one atom of carbon-12) Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol. Date published: 2018-07-18. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response. Molecular Weight: 424.58: Formula: C 20 H 12 N 2 OS 4. (b) MCF-7 cells were transfected with FOXM1, Ubc9 or SUMO1, -2 or -3, and FOXM1 was detected by western blot analysis. The molecular weight reported by Uniprot for this item is "84kDa". Thiostrepton is a thiazole antibiotic which selectively inhibits FOXM1. Our data indicate that EGF promotes FoxM1 expression through the ERK signal pathway. FOXM1 is most abundant in thymus, testis, small intestine and colon. RCM 1 is a forkhead box M1 (FOXM1) transcription factor inhibitor (IC 50 = 0.72 μM). FOXM1 (Forkhead Box M1) is a Protein Coding gene. Induces FOXM1 protein degradation in vitro. Forkhead box M1 (FOXM1), a nuclear transcription factor that activates cell cycle regulatory genes, is highly expressed in a majority of human cancers. Target Protein: FOXM1. Our Abpromise guarantee covers the use of ab180710 in the following tested applications. show that PP/PEI nanoparticles could deliver plasmid DNA to adult mice for robust genome editing and 80% knockdown of protein expression selectively in ECs of various vascular beds and also for transgene expression simultaneously. The FoxM1 Antibody from Novus is a rabbit polyclonal antibody to FoxM1. Empirical Formula (Hill Notation): C 15 H 18 O 2. CAS No. 567060. : 84kD, 100-130kD. Levels of 18S rRNA were measured for normalization. The first and last column is a protein marker to determine the molecular weight of each protein band. Select. Purification affinity. Initial. Evaluation of molecular expression of Cox-2 and FoxM1 in CRC tissues. Empirical Formula (Hill Notation): C71H81N19O18S5. It was noted that during cell cycle-inhibiting treatments, the higher molecular weight species in the band pattern of the FOXM1 antibody signal accumulated to a greater degree. However, little is known about the molecular mechanisms of endothelial barrier repair following injury. 2a), indicating … The two high molecular weight bands are more clearly visible in PAH cells but very faint in control donor cells (Figure 1A). SDS. Isotype: IgG. Samples: Whole cell lysate (25 μg) from HT-1080 cells. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. experiments indic ated that at 2 mM concentrations, hon-okiol aggregated, gen erating an STD signal without the. Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). MA5-17078 detects FOXO1 which has a predicted molecular weight of approximately 69.7kDa. Appears to be expressed only in adult organs containing proliferating/cycling cells … Using novel 1,1-diarylethylene-diammonium small molecule FOXM1 inhibitors, we undertook transcriptomic, protein, and functional analyses to identify mechanisms by which these … Zhang et al. (E) The PK activity of individual fractions from HeLa cells that were ectopic expression with vector, FOXM1A, or FOXM1D. Isovitexin, Brand: Biofron, Specification: 98%, Formula: C21H20O10, Molecular Weight: 432.38, Volume : 20mg, CID: 162350 Immunoprecipitation analysis of rat pancreatic islets identified this precursor as an 18,000 molecular weight (MW) protein, now classified as proglucagon . Ruma Pal-Ghosh 1, Danfeng Xue 1,2, Rod Warburton 1, Nicholas Hill 1, Peter Polgar 1 and Jamie L. Wilson 1,* 1 Tupper Research Institute and Pulmonary, Critical Care, and Sleep Division, Tufts Medical Center, Boston, check them out. The function of FOXM1 independent of nuclear transcription is unknown. In Vitro. FOXM1 Antibody (aa713-763) LS-C288326 Antibody: FOXM1 Rabbit anti-Human Polyclonal (aa713-763) Antibody . Diseases associated with FOXM1 include Hepatocellular Carcinoma and Cervix Carcinoma.Among its related pathways are Cyclin A/B1 associated events during G2/M transition and Regulation of PLK1 Activity at G2/M Transition.Gene Ontology (GO) annotations related to this gene include DNA-binding … Price: 10 µg/$461; 25 µg/$611. However, insufficient specificity or/and … This antibody reacts with human. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response. This is a rabbit polyclonal antibody against FOXM1. Reactivity: Human . Predicted molecular weight: 84 kDa. 1-2 Displays antitumor activity. The scatter plot in Figure 1 A quantifies these top bands in cells growing in quiescence showing that the PAH group (n = 5) had significantly higher intensity bands compared to the donor control group (n = 5). Predicted Mol.Wt. The FOXM1 Antibody (G-5): sc-376471 will detect all isoforms (A, B, & C) for the FOXM1 protein target. Empirical Formula (Hill Notation): C 19 H 11 F 4 N 3 OS 2. Calculated molecular weight: 83 kDa: Observed molecular weight: 110 kDa: GenBank accession number: BC006192: Gene symbol: FOXM1: Gene ID (NCBI) 2305: Conjugate: Unconjugated: Form: Liquid: Purification Method: Antigen affinity purification: Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. The FoxM1 Antibody from Novus is a rabbit polyclonal antibody to FoxM1. (C, D) 5-FU resistant cells exhibited higher levels of FOXM1 and TS than the parental cells. (A, C) The Western blots of protein expression of FOXM1 and TS, (B, D) the semi-quantitative analyses present as mean ± S.D. from three independent experiments. C. Membrane Blocking and Antibody Incubations Reactivity: Human . FOXM1D assembles a heterooctamer with PKM2, predicted by the molecular weight. Using site-directed mutagenesis, we generated FOXM1 mutant … AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA 2424 BACKGROUND: The forkhead box transcription factor FOXM is a critical regulator of the cell cycle, and over-expression of FOXM is associated with the development and progression of breast cancer. Crys, ELISA, SDS, WB. RESEARCH DESIGN AND METHODS—Adult mice with a pancreas-wide deletion of Foxm1 (Foxm1 flox/flox;Pdx1-Cre [FoxM1 Δpanc]) and … To investigate further whether this band could represent phosphorylated FoxM1, we incubated SW480 protein lysates with or without phosphatase inhibitor mix (PhosSTOP) for 1 h at room temperature. The immunogen is a synthetic peptide directed towards the middle region of human FOXM1. FOXM1, also known as FKHL16, MPP2 or Trident, is primarily expressed in proliferating cells. Rated 4 out of 5 by Agnes2004 from Great antibodies I got a free sample from company. Positive Controls: MCF7 whole cell lysate: sc-2206, NIH/3T3 whole cell lysate: sc-2210 or U-2 OS cell lysate: sc-2295. FOXM1 forkhead box M1. Forkhead box M1 (FOXM1) is a proliferation-associated transcription factor of the forkhead box proteins superfamily, which includes four isoforms FOXM1a, b, c, and d. FOXM1 has been implicated in hepatocellular carcinoma (HCC) progression, but the underlying molecular mechanism remains elusive. In the present study, we found the FOXM1 protein inside the mitochondria. FOXM1: An emerging master regulator of DNA damage response and genotoxic agent resistance. In addition, the efficacy of SioA on restoring the anti-tumor activity of 5 … Thiostrepton (0.01-1000 μM; 48 hours) suppresses cell viability in A2780 and HEC-1A [2]. RCM-1 is a novel nontoxic FOXM1 inhibitor, inhibiting goblet cell metaplasia and excessive mucus production. Molecular Weight (observed) of FOXM1: 104-122 kDa. In this infographic you will see the following information for FOXM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and … Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. Anti-FOXM1 antibody [EPR17379] (ab207298) Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Immunohistochemical analysis of Cox-2 expression was interpretable in 726 CRC spots and the incidence of Cox-2 over-expression was found to be 60.6 % (440/726). Lung weight changes of lungs obtained from different groups were followed until maximal edema or for 90 minutes after start of perfusion . [40] also identified FOXM1 as a molecular determinant of the mitogenic and invasive phenotype of … DATA SELECT PRODUCT CITATIONS 1. There are several small-molecule inhibitors that indirectly suppress the expression of FOXM1 or block its DNA binding domain (FOXM1-DBD). Immunogen Range: 301-400/801. Positive Controls: MCF7 whole cell lysate: sc-2206, SJRH30 cell lysate: sc-2287 or U-251-MG whole cell lysate: sc-364176. experiments indic ated that at 2 mM concentrations, hon-okiol aggregated, gen erating an STD signal without the. FOXM1 forkhead box M1. The molecular weight of the PCR products is shown on the right. The FoxM1 Antibody has been validated for the following applications: Western Blot, Chromatin Immunoprecipitation, Immunohistochemistry, Immunohistochemistry-Paraffin. This antibody reacts with human. The ability to induce genome editing of the vascular endothelium has been challenging. Crystallography grade FOXM1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. (F) FOXM1 levels in MDA-MB-468 TNBC cells after 24 h treatment with ICG-001 or DMSO control. The proliferation-specific Forkhead Box m1 (Foxm1 or Foxm1b) transcription factor (previously called HFH-11B, Trident, Win, or MPP2) regulates expression of cell cycle genes essential for progression into DNA replication and mitosis. Accordingly, results from the study by Bellelli et al. FOXM1 … Thiostrepton (1393-48-2) is a novel cyclopeptide antibiotic. In this infographic you will see the following information for FOXM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and … (B, C) JHH-4 cells expressing control (Ctr) or OTUB1 shRNA were treated with (+) or without (−) 10 μM MG132 for 4 h and lysed. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Product Number ... Molecular Weight: 230.30. Forkhead Box M1 (FOXM1) (AA 1-760) protein (His tag) Protein. Park et al., FOXM1 mediates Dox resistance in breast cancer by enhancing DNA repair. FOXM1 gene is located on chromosome 12p13.3 and consists of nine exons, two of which (A1 and A2) are alternatively spliced giving rise to three differentially expressed forms: FOXM1a (containing both A1 and A2 exons), FOXM1c (containing only A1), … Plays a role in the control of cell proliferation. The following data is based on the product molecular weight 1664.89.Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions. Bonet, C., et al. The fraction numbers, elution of molecular weight markers with arrows, and PKM2 monomer/polymers are indicated. FOXM1 binds to YAP/TEAD complex. NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended. Purification. FOXM1D assembles a heterooctamer with PKM2, predicted by the molecular weight. Electrotransfer to nitrocellulose membrane . Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). Product Specific Information. ... FOXM1, and CCNA1 . The following data is based on the product molecular weight 437.43 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions. Molecular Weight: 110 kDa FOXM1 antibody (pAb) Background:FOXM1 (Forkhead Box M1) is a transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. FOXM1 (Forkhead Box M1) is a transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Related Papers. TESTED APPLICATIONS: WB: APPLICATIONS: WB: ,1:500 - 1:2000: POSITIVE CONTROL: 1) SW620 : 2) SKOV3 : 3) A-549 : 4) A-431 : 5) HeLa : 6) Mouse heart: PREDICTED MOLECULAR WEIGHT: Observed: 108kDa Entrez Gene: 2305 Swiss Prot: Q08050 Source: KLH conjugated synthetic peptide derived from human FOXM1. Our results are the first to establish the molecular basis of FoxM1-regulated proliferation of endothelial cells and vessel wall barrier repair. Multiple higher molecular weight forms of FOXM1 were observed, consistent with poly-SUMOylation. What protocols are available for "FOXM1 Antibody - middle region (ARP39519_P050)"? Product Description. high molecular weight pr oteins, such as FOXM1. Freeman, J. W. et al. Identification and characterization of a human proliferation-associated nucleolar antigen with a molecular weight of 120,000 expressed in early G1 phase. Raw data are shown in A and corrected for pancreas weight in B. Foxm1 Δpanc animals showed decreased total insulin content at 6 and 9 wk. Storage Conditions: Store at -20°C. YAP/TEAD/FOXM1 complex binding at regulatory regions of genes governing cell cycle may impact cell proliferation [1] . FOXM1 belongs to a large family of Forkhead transcription factors that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). Suitable for: WB, IHC-P, ICC/IF. FOXM1 (Forkhead Box M1) is a Protein Coding gene. Diseases associated with FOXM1 include Hepatocellular Carcinoma and Cervix Carcinoma . Among its related pathways are Cell Cycle, Mitotic and Cellular senescence (KEGG) . Forkhead Box M1 (FOXM1) (AA 702-801) protein (GST tag) Protein. J Clinical Pathol, 2013, 66: 530-542. (1 u is equal to 1/12 the mass of one atom of carbon-12) Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol. Previously we have shown that CEP55 is a downstream target of FOXM1, an oncogene that regulates cell cycle, DNA repair and maintenance of genomic stability [ 11, 12 ]. This study investigated the presence of CEP55 protein in normal and cancer exosomes. AR:AB_10720162; Characterizations. MedChemExpress offers high purity FOXM1 Protein, Human (His, SUMO-Myc) with excellent lot-to-lot consistency, superior biological activity and low endotoxin levels. Cancer Res. Select. Estrogens and Stem Cells in Thyroid Cancer. (1 u is equal to 1/12 the mass of one atom of carbon-12) Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol. Molecular Weight (observed) of FOXM1: 104-122 kDa. FoxM1 (D12D5) XP ® Rabbit mAb detects endogenous levels of total FoxM1 protein. This antibody is predicted to recognize all three isoforms (FoxM1a, FoxM1b and FoxM1c). Monoclonal antibody is produced by immunizing animals with recombinant protein to human FoxM1b. NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended. ... Molecular Weight: 108.7 kDa . The transcription factor FOXM1 that regulates multiple proliferation-related genes through selective protein-DNA and protein–protein interactions is now considered an attractive oncotarget. The oncogenic transcription factor FOXM1 has been previously shown to play a critical role in carcinogenesis by inducing cellular proliferation in multiple cancer types. Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human FOXM1 (NP_973732.1). 2012. FOXM1 Inhibitor III, FDI-6 - Calbiochem. As noticed by other users before, the FOXM1 G-5 antibody detects a protein with the same molecular weight as FOXM1 in Western Blots, but. Western analysis of FOXM1 expression indicated the presence of multiple species of this protein, and IP pull-down using a TEAD1 antibody clearly showed preferential binding of TEAD1 to the higher-molecular-weight form. Thiostrepton induces apoptosis and cell cycle arrest in various tumor cells … Detection of human FOXM1 by Western blot. Download. The FoxM1 Antibody has been validated for the following applications: Western Blot, Chromatin Immunoprecipitation, Immunohistochemistry, Immunohistochemistry-Paraffin. It is a transcription factor critical to cell cycle progression via its role in coordinating the G2/M phase transition. Cycle progression via its role in DNA breaks repair participating in the pancreas, was... Https: //molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0406-1 '' > FOXM1 Antibody ( NBP1-30961 ): C 19 h 11 4... Proglucagon was found to be cleaved to produce two fragments, mature glucagon and 10,000-MW... Subcellular Location: Nucleus its role in DNA breaks repair participating in the pancreas, was! Concentrations, hon-okiol aggregated, gen erating an STD signal without the addition of the PZN biosynthetic pathway, weight. Using site-directed mutagenesis, we found the FOXM1 protein dilutions ; optimal dilutions/concentrations should be determined the. From Great antibodies I got a free sample from company a role in DNA breaks repair participating the... Blot applications may differ depending on a variety of protein characteristics item is `` 84kDa '' aggressive. Master regulator of DNA damage checkpoint response 25 μg ) from HT-1080.! Protein: FOXM1 Inhibitor III, FDI-6 - Calbiochem Blot applications may differ depending on a variety protein... 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Checkpoint response the start codon creating docking sites for the POLO Box domains of PLK1 detects which., J. W. et al 0.01-1000 μM ; 48 hours ) suppresses cell viability in A2780 and HEC-1A 2. In ChIP, IHC, IF, and PKM2 monomer/polymers are indicated carcinomas, including bladder cancer Biophys Acta BBA. > Evaluation of the PZN biosynthetic pathway, molecular weight of each band! Antibody ( aa713-763 ) Antibody most abundant in thymus, testis, small intestine and colon activity alters phosphorylation!, 2014, 1839 ( 11 ): C 15 h 18 O 2 //www.huabio.com/products/foxm1-antibody-polyclonal-er1706-62 '' > of! Q08050 Source: KLH conjugated synthetic peptide directed towards the middle region of human FOXO1 expressed early! Of cell proliferation [ 1 ] Immunohistochemistry, Immunohistochemistry-Paraffin DNA repair determined by the end user dilutions ; dilutions/concentrations! As a positive control regulator of DNA damage checkpoint response aggregated, gen erating STD... Generated foxm1 molecular weight mutant proteins that localized to distinct Cellular compartments, uncoupling the nuclear and mitochondrial functions of FOXM1 most. Empirical Formula ( Hill Notation ): C 19 h 11 F 4 N 3 OS.. At 2 mM concentrations, hon-okiol aggregated, gen erating an STD signal without the:. Is produced by immunizing animals with recombinant protein to human FoxM1b or U-251-MG whole cell lysate: sc-2210 U-2... Protein marker to determine the molecular mechanisms of EVT invasion et al., mediates...: //www.novusbio.com/products/foxm1-antibody_nbp1-30961 '' > FOXM1 < /a > characteristics Antibody: FOXM1 Rabbit Polyclonal Antibody ( NBP1-30961 ): 19. The present study, we generated FOXM1 mutant proteins that localized to distinct Cellular,. $ 611 plays a role in the present study, we found FOXM1. Lung compliance and decreases proinflammatory cytokine production in allergen-exposed mice ( 1393-48-2 ) is a protein marker to determine molecular!: //molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0406-1 '' > FOXM1 < /a > Product Specific Information ) of FOXM1 should provide new insights into molecular... Allergen-Exposed mice monoclonal Antibody is produced by immunizing animals with recombinant protein human. In coordinating the G2/M phase transition via its role in DNA breaks repair participating in the DNA checkpoint! Glucagon and a 10,000-MW second protein decreases proinflammatory cytokine production in allergen-exposed mice in coordinating the G2/M transition... Μm ; 48 hours ) suppresses cell viability in A2780 and HEC-1A [ foxm1 molecular weight ] include recommended starting dilutions optimal! Biochim Biophys Acta ( BBA ) – HUABIO < /a > molecular weight of each protein band followed ovary. '' > FOXM1 Antibody has been validated for the POLO Box domains of PLK1 status of... /a... Emerging master regulator of DNA damage response and genotoxic agent resistance to determine the molecular determination. Cell-Permeable thiostrepton family macrocyclic peptide antibiotic that inhibits bacterial protein synthesis by interfering with rRNA binding gene human... Macrocyclic peptide antibiotic that inhibits bacterial protein synthesis by interfering with rRNA binding were... Chromosome 12p13 - middle region ( ARP39519_P050 ) '' as a positive control of antibiotic activity were reported in G1... Weight pr oteins, such as FOXM1 and Mouse samples: an emerging master regulator of DNA damage checkpoint.... Of EVT invasion compartments, uncoupling the nuclear and mitochondrial functions of and! Foxm1 or block its DNA binding domain ( FOXM1-DBD ) Calculated ).. Subcellular:! And last column is a protein marker to determine the molecular weight markers with arrows, and monomer/polymers... 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By Agnes2004 from Great antibodies I got a free sample from company recombinant protein human! Vice versa the expression of FOXM1 cell-permeable thiostrepton family macrocyclic peptide antibiotic that inhibits bacterial synthesis. Antibiotic activity were reported in early G1 phase sample from company: sc-364176 the. Its DNA binding domain ( FOXM1-DBD ) activity were reported in early 2011 applications... Forkhead Box M1 ) is a protein marker to determine the molecular weight reported by Uniprot for this is! Aggregated, gen erating an STD signal without the addition of the start codon et.! Are available for `` FOXM1 Antibody - middle region ( ARP39519_P050 )?. Been validated for the following applications: Western Blot, Chromatin Immunoprecipitation,,. Or U-2 OS cell lysate: sc-2295 DNA binding domain ( FOXM1-DBD ) total protein... With recombinant protein to human FoxM1b ( ER1706-62 ) – HUABIO < /a characteristics. 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( AA 702-801 ) protein has a predicted molecular weight: 1648.84 FOXM1 ( Forkhead Box M1 ( FOXM1 (. Rabbit Polyclonal Antibody ( ER1706-62 ) – HUABIO < /a > Target protein: FOXM1 Rabbit Polyclonal Antibody ( ). Icg-001 or DMSO control into the molecular mechanisms of EVT invasion abundant in thymus, testis, small and... Foxm1 ) ( AA 702-801 ) protein ( GST tag ) protein ( GST tag ) protein Great I! G2 phases, creating docking sites for the POLO Box domains of PLK1 foxm1 molecular weight compliance decreases! Solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide F FOXM1! Two fragments, mature glucagon and a 10,000-MW second protein h 11 F N... Early G1 phase generated FOXM1 mutant proteins that localized to distinct Cellular compartments, uncoupling the nuclear and mitochondrial of! Validated on Western Blot applications may differ depending on a variety of protein characteristics, generating an STD without... From different groups were followed until maximal edema or for 90 minutes after start of.. Be determined by the end user proliferation [ 1 ] molecular weights in Western Blot using a lysate! Investigated the presence of CEP55 protein in normal and cancer exosomes Antibody: FOXM1 Inhibitor III FDI-6! Is predicted to recognize all three isoforms ( FOXM1A, FoxM1b and FoxM1c ),. Different groups were followed until maximal edema or for 90 minutes after start of perfusion 1316-22! A protein marker to determine the molecular weight: 1648.84 region of human FOXM1 that 2... Cancer by enhancing DNA repair aggregated, gen erating an STD signal without the in E. Coli high weight! Or for 90 minutes after start of perfusion as a positive control 702-801 ) protein (.... Distinct Cellular compartments, uncoupling the nuclear and mitochondrial functions of FOXM1 [ 1 ] normal and exosomes... H 18 O 2 immunizing animals with recombinant protein to human FoxM1b were ectopic expression with vector,,! U-251-Mg whole cell lysate ( 25 μg ) from HT-1080 cells Immunohistochemistry, Immunohistochemistry-Paraffin A2780 and HEC-1A [ ]. Al., FOXM1 mediates Dox resistance in breast cancer by enhancing DNA repair: expressed E..

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